Assessing the performance of Moghani crossbred lambs derived from different mating systems with Texel and Booroola sheep

In our ongoing project, which focuses on the introgression of Booroola/FecB gene and the myostatin (MSTN) gene into purebred Moghani sheep, we assessed the performance of second-generation Moghani crossbreds such as second crossbreds (F2) and initial backcross generation (BC1). These crossbreds were generated through different mating systems, including in-breeding, outcrossing, first paternal backcrossing (PBC1), and first maternal backcrossing (MBC1). Notably, F2 strains exhibited lean tail, woolly fleece and a higher percentage of white coat color compared to BC1. The impact of mating systems and birth types on pre-weaning survival rates was found to be statistically significant (P < 0.0001), with singleton offspring resulting from paternal backcross showing a particularly substantial effect. The F2 crossbred lambs carrying the Booroola gene did not show a statistically significant difference in survivability compared to those carrying the MSTN gene, implying the Booroola prolificacy gene had no significant impact on survival outcomes. However, the occurrence of multiple births had a significant negative impact on lamb survival (P < 0.0001). The PBC1 sheep strains, specifically Texel Tamlet ram strains carrying the MSTN mutation, exhibited superior growth rates compared to others (P < 0.05). Interestingly, the MSTN mutation in the homozygous variant genotype significantly impacts growth rate before weaning compared to other genotypes and pure Moghani sheep (P < 0.05). In conclusion, this study objectively underscores the pivotal role of genetic factors, specifically through strategic mating systems like paternal backcrossing, in enhancing desired traits and growth rates in Moghani sheep, thereby contributing valuable insights to the field of sheep breeding programs.


Introduction
Mating systems play a crucial role in influencing the performance and genetic traits of livestock.Researchers have investigated a range of mating systems, including pure-breeding, crossbreeding, outcrossing, and backcrossing, to evaluate their effects on vital characteristics such as growth traits, lamb survival, and ewe prolificacy [1][2][3][4][5][6].Crossbreeding with major gene carriers, using local or exotic germplasm, and genomic introgression offer promising pathways to achieve substantial genetic gains [7].Successful sheep crossbreeding programs often involve various mating systems, such as F1 crosses, three-way crosses, and composite breeds [8].This approach accelerates genetic progress, overcoming challenges associated with direct selection for quantitative traits, leading to more productive and profitable sheep farming operations.
Mature body size in sheep is known to be influenced by a higher degree of polygenic factors when compared to other domesticated species [9].This means that many genes, each with a small effect, contribute to mature body size.This complex genetic nature presents a significant challenge, rendering classical breeding methods alone insufficient in achieving desired outcomes [10].
The Moghani sheep is a local breed in Iran that is raised primarily for meat production.It is known for its large fat tail and lower prolificacy, but it also has the advantage of being able to reproduce out of season [4].However, the Moghani sheep faces challenges in terms of economic profitability.Numerous studies have shown that the genetic progress for growth and reproductive traits in this breed is slow, due to low heritability estimates [31][32][33][34].
Herein, we launched an introgression project to enhance the productivity of Moghani sheep through strategic crossbreeding with high-yielding sheep breeds, specifically Texel and Booroola sheep.These breeds possess crucial genetic factors known for enhancing traits such as muscularity and prolificacy.The comprehensive introductory details and outcomes of the F1 crosses, including Booroola Merino×Moghani (BMM), Booroola Romney×Moghani (BRM), Texel Tamlet×Moghani (TTM), and Texel Dalzell×Moghani (TDM), have been extensively documented in our prior publications [4,6].In our ongoing project, we bred secondgeneration Moghani crossbreds using various mating systems, including in-breeding, outcrossing, and backcrossing.This study specifically focuses on a comprehensive comparison of growth performance, fat-tail traits, and lamb coat colors between purebred Moghani sheep and second-generation crossbreds.Additionally, we investigated the impact of distinct genotypes of introgressed genes, particularly prolificacy Booroola/FecB and hyper-muscularity myostatin (MSTN g+6223G>A), on the performance of these second-generation Moghani crossbreds.It's important to note that, despite our meticulous examination of these traits, we unfortunately couldn't evaluate prolificacy due to the lack of available records.Acknowledging the significance of this parameter, we plan to include it in future investigations as more data becomes accessible.

Ethics statement
The data collection formats and procedures employed in this study underwent thorough review and approval by the Animal Care and Use Committee at the Agricultural Biotechnology Research Institute (ABRII) in Karaj, Alborz, Iran.The committee granted approval for all procedures and activities involving animals, ensuring strict adherence to local guidelines.The study exclusively relied on data obtained from live sheep at the breeding facility of Jovain Agricultural & Industrial Corporation in Jovain, Razavi Khorasan, Iran.It is important to note that no invasive procedures were conducted, and the animals were closely monitored by researchers.The study did not involve anesthesia, euthanasia, or animal sacrifice.

Management of housing conditions, feeding regimens, and health monitoring
The animals were raised at the breeding facility of Jovain Agricultural & Industrial Corporation in Jovain, Razavi Khorasan, Iran (Jovain, Razavi Khorasan, Iran.Latitude: 36.655297/N36˚39' 19.06800@, Longitude: 57.423406/57˚25' 24.26100@).Jovain County experiences an average annual rainfall of approximately 272 mm, with a mean daily temperature range of 17.8 to 29.5˚C, characterizing it as a moderately warm climate zone.A semi-intensive management system, characterized by a moderate amount of production inputs, was employed for animal care.The animals were permitted to graze or browse on natural pasture for approximately six hours during the daytime.Additionally, they received a supplementary diet of 0.10 to 0.40 kg concentrate mixture per day, consisting of alfalfa barn, maize silage, and salt.The amount varied based on factors such as age, physiology, and sex.Housing arrangements were organized according to sex, physiological status, and health status.Animals had access to water ad libitum and were subjected to vaccinations against prevalent diseases in the area.Regular treatments, deworming, and scheduled spraying were conducted to maintain their health.Each kid was assigned a unique identifying number, and their birth weight was recorded within 24 hours of birth.Kids were kept indoors with their dams for three to seven days, after which dams were moved outdoors, and kids were allowed to suckle three times a day until reaching the weaning age of 90 days.Animal care staff performed routine health assessments to ensure the overall well-being of the animals.

Lambing information
In the context of in-breeding, a total of 25 lambs were born alive within the first week of birth, derived from a group of 39 lambs born to 25 uniparous F1 ewes aged 1 year.This group encompassed 14 F2 Booroola lambs (BRMBRM-2 and BMMBMM-2) as well as 15 F2 Texel lambs (TTMTTM-2 and TDMTDM-2).Turning to the subject of outcrossing, 40 live lambs were delivered among 48 offspring from 31 multiparous F1 ewes aged 2 years.Among these were F2 Texel lambs, exemplified by TDMTTM-2 and TTMTDM-2.In the case of PBC1, there was a count of 435 live lambs born from a pool of 438 lambs, all from 385 multiparous purebred Moghani ewes aged 3 years.The lambs were identified as TDMM-2, TTMM-2, BMMM-2, and BRMM-2.Shifting focus to MBC1, 34 live lambs were welcomed into the world among 36 offspring from 28 multiparous BMM and BRM ewes aged 2 years, showcasing the presence of MBRM-2 and MBMM-2 lambs.

Records and data management
The growth performance of purebred Moghani, and second (F2 and BC1) generations of crossbred lambs were evaluated.Before the analysis, we adjusted weights to corresponding 90 (3-months age), and 180 (6-months age) days respectively representing adjusted 3-months weight (W3 adj ) and adjusted 6-months weight (W6 adj ), using the following formulas: Where, BW = birth weight (kg), W3 = weight at 3 months of age (kg), W6 = weight at 6 months of age (kg), D1 = number of days between birth day and the 3-months weighing, D2 = number of days between the 3-month and 6-months weighings, Diff1 = difference of weights from birth to 3 months (kg), Diff2 = difference of weights from 3 to 6 months (kg), ADWG1 = average daily weight gain from birth to 3 months (gr), and ADWG2 = average daily weight gain from 3 to 6 months (gr).Additionally, fat-tail traits at 6 months of age were documented, encompassing tail/fat tail type, fat-tail height (FTH, in centimeters), and fat-tail width (FTW, in centimeters).Furthermore, morphometric of the lamb coat colors were collected in five coat colors types including white, brown, light-brown, strong brown, and black.

Extraction of DNA and genotyping through PCR-RFLP method
Blood sampling was collected two to three months after birth.Genomic DNA was extracted using the procedure closely followed the methodology as previously described [35].The PCR-RFLP genotyping procedures for BMPR1B mutation OAR6:g.29382188A>G and the MSTN mutation OAR2:g.118150665G>Awere conducted following the methods outlined in [4].

Statistical analysis
The frequency of colors, mating systems, sex, introgressed gene, and type of birth were compared based on two-way chi-square tests with a significance level of 5%, using the PROC FREQ procedure in SAS Version 8.2 [36].
The analysis of growth traits utilized the general linear model (GLM) procedure implemented in SAS.The determination of significance (P < 0.05) was conducted through Duncan's multiple range test.The analysis employed the following multivariate models: where: y ijklmnopqr is the vector of observation of the i th animal within the j th sire breed, k th dam breed, l th sheep strain, m th type of birth, n sex category, o th mating system, p th genotype category, and q th tail type.
μ is the overall mean, B j is the effect of the j th sire breed (j = Moghani, TTM, TDM, BMM, BRM), D k is the effect of the k th dam breed (k = Moghani, TTM, TDM, BMM, BRM), C l is the effect of the l th sheep strain (l = Moghani, BRMBRM-2, BMMBMM-2, BMMM-2, BRMM-2, MBRM-2, MBMM-2, TTMTTM-2, TDMTDM-2, TDMTTM-2, TTMTDM-2, TDMM-2, TTMM-2), T m is the effect of m th type of birth (m = 1, 2, 3, 4), S n is the effect of n th sex (n = male and female), M o is the effect of o th mating system (o = in-breeding, outcrossing, paternal backcrossing, maternal backcrossing, and pure-breeding), G p is the effect of p th genotype (GG, GA, AA for MSTN mutation and AA, AG, GG for BMPR1B mutation), L q is the effect of q th tail type (q = tail or fat-tailed), (B×D) jk is the interaction effect between j th sire breed and k th dam, (C×G) lp is the interaction effect between l th strain and p th genotype, (M×G) op is the interaction effect between o th mating system and p th genotype.

RFLP genotyping results
The RFLP genotyping analysis of the MSTN mutation (OAR_v.It is noteworthy that lambs with the homozygous variant genotype for both genes have exclusively been discovered in progenies resulting from a combination of in-breeding and outcrossing systems.A study by Daetwyler et al. [37] revealed a negative link between inbreeding rates and heritability.This is due to reduced genetic diversity caused by inbreeding.Hence, it underscores the necessity for precise breeding management to regulate these genetic variations.

Phenotype and morphometric characteristics
The initial observable traits in the F1 crossbred lambs were a slender tail, a white coat, and a woolly white fleece.The purebred Moghani sheep, on the other hand, had a substantial fat-tail and a light-brown fleece that was either woolly or hairy [4].Consistent with the results reported by Khaldari et al. [38,39], the mating of lean-tailed rams with fat-tailed ewes resulted in the birth of F1 crossbred lambs with slender tails, regardless of gender.Moving on to the second-generation of crossbred lambs, twelve strains, including TTMTDM-2, TTMTTM-2, TDMTDM-2, TDMTTM-2, BRMBRM-2, BMMBMM-2, TDMM-2, TTMM-2, BRMM-2, BMMM-2, MBMM-2, and MBRM-2, generated through different mating systems, have been shown in Fig 3 .The offspring resulting from both in-breeding and outcrossing exhibited a lean tail, and a woolly fleece (Fig 4A -4D).In the context of backcrossing progeny, these lambs displayed varying from short (including fat-rumped) to large fat-tails, as well as a woolly/hairy fleece (Fig 4E).The shape and size of a sheep's tail play a crucial role in their genetics and have important implications for their domestication, ability to thrive in various environments, productivity, and animal welfare [40].At present, the fat-tail phenotype is not favorable for inclusion in breeding programs in Iran, as it presents several adverse consequences that are likely to impact aspects such as animal mobility, mating, food efficiency, and breeding expenses [4,11].

Preweaning lamb survival
The data pertaining to preweaning lamb survival across various variables such as mating systems, sex, introgressed gene, and type of birth has been detailed in Table 2.The mating systems and type of birth significantly affected lamb's pre-weaning survival rates (refer to Table 2, P < 0.0001).Notably, singleton offspring resulting from paternal backcross exhibited a substantial impact.Further, females displayed a slightly improved survival rate compared to males, the observed differences did not reach statistical significance (refer to Table 2, P > 0.05).Consequently, it can be inferred that the survival rates of females and males are similar.These findings align with prior researches [5,42] that also noted fluctuations in lamb survival rates.Our study corroborates these observations and indicates that the presence of introgressed MSTN and Booroola genes does not exert a noteworthy impact on lamb survivability (see significant difference in survivability compared to lambs carrying the MSTN gene, as indicated in Table 2.The findings suggest that the Booroola prolificacy gene does not have a detrimental impact on lamb survivability.However, it is important to highlight that we observed a decline in lamb survival during cases of multiple births (refer to Table 2).This observation aligns with previous unfavorable findings when the Booroola mutation was introduced into Australian [43] and American [44] sheep breeds.In these studies, lamb mortality notably increased among highly prolific ewes managed in extensive conditions.This occurrence can be linked to the counteractive correlation between heightened prolificacy and lamb survival rates, combined with an increased susceptibility to pregnancy toxemia in ewes [8].The ability of fetuses to resist hypoxia is critically significant in pregnancies with multiple fetuses, as hypoxia can have a significant impact on fetal survival and birth weight [45].

Effect of birth type on growth performance of lambs
The impact of birth type on the growth performance of second generations of crossbred lambs (F2 and BC1) is presented in Table 3. Pre-weaning weights, such as birth weight (BW) and weaning weight (W3 adj ), exhibited a significant difference.Quadruplets and triplet-born lambs displayed notably lower weights compared to twins and single-born lambs (P < 0.0001, see Table 3).In contrast, post-weaning, triplets exhibited a faster growth rate than single-born, twins, and quadruplet-born lambs, with an increase of 255.06 g/d (P < 0.9129, refer to Table 3).These findings align with the results of our prior study on F1 crossbreds [4].A study has demonstrated that ewes rearing triplets produce 21% more milk and exhibit greater feedto-milk conversion efficiency when compared to ewes of similar weight rearing twins [46].This is likely attributable to the influence of the number of lambs nursed on ewe lactation.In contrast, McHugh et al. [47] found that lambs born and reared as triplets exhibited a notably slower growth rate of 299 g/d.Additionally, in a study examining the impact of the Booroola gene on the growth performance of Garole × Malpura sheep, Kumar et al. [48] found that the type of birth had a significant effect (P < 0.01) on body weight from birth to 12 months of age.Notably, single-born lambs exhibited a significantly higher body weight (P < 0.01) compared to twins and triplets within the same age range.Furthermore, the type of birth had a significant impact (P < 0.01) on the average daily weight gain before weaning, while it did not significantly affect the average daily weight gain after weaning.

Effects of sex and mating systems on growth performance of lambs and fattail traits
In the present study the female lambs have significantly lower growth rate compared to males (Table 4).Moreover, female lambs have greater FTH (18.10 cm vs. 15.48 cm) but lower FTW (15.53 cm vs. 18.12 cm) than male lambs (P < 0.05).Fig 1 illustrates the use of four distinct mating systems (Fig 1C -1F) in producing crossbred lambs of F2 and BC1.Notably, the offspring resulting from paternal backcrosses exhibited significantly greater birth weights compared to those from the other mating systems (P < 0.0001, Table 5).While lambs born through pure-breeding, in-breeding, and outcrossing displayed higher average daily weight gain before weaning (ADWG1) when contrasted with the backcrossed lambs, the situation shifted post-weaning.After weaning, the lambs born through backcrossing demonstrated a significantly higher growth rate (ADWG2) compared to the others (P < 0.0001, Table 5).To enhance the growth rate, we have determined that paternal backcrossing is the optimal strategy for the introgression of major genes into the Moghani pure breed.When it comes to fat-tail traits, lambs born from pure-breeding Moghani sheep exhibited significantly greater FTH and FTW compared to lambs born from various mating systems, including in-breeding, outcrossing, paternal backcrossing, and maternal backcrossing (P < 0.0001, Table 5).

Effects of progeny strains on growth performance of lambs and fat-tail traits
In this study, strains resulting from paternal backcrossing, namely TTMM-2, TDMM-2, BMMM-2, and BRMM-2, exhibited greater birth weight (BW), as well as W3 adj and W6 adj measurements, and growth rates when compared to other sheep strains (P < 0.05, Table 6).Conversely, strains derived from maternal backcrossing systems, such as MBRM-2 and MBMM-2, displayed lower body weight compared to pure Moghani sheep before weaning.However, after weaning, they exhibited significantly higher growth rates (P < 0.05, Table 6).Among the various strains assessed, TTMM-2 exhibited superior characteristics, including higher BW, W3 adj , W6 adj , Diff2, and ADWG2 in comparison to other strains.However, Moghani pure sheep displayed greater Diff1 and ADWG1 than TTMM-2, although the difference was not statistically significant (refer to Table 6).TTMM-2 originated from the  [11].Despite the BRMBRM-2, BMMBMM-2, TTMTTM-2, and TDMTDM-2 sheep strains, resulting from inbreeding systems among F1 crossbred lambs, displaying lower birth weights, the Texel sheep strains, including TTMTTM-2 and TDMTDM-2, demonstrated significantly higher W3 adj and ADWG1 when compared to other sheep strains (P < 0.05, see Table 6).These findings suggest that these inbred Texel sheep strains perform better than other sheep strains in terms of reaching the weaning stage.

Association of introgressed genes with growth traits
Comparing the results of different genotypes of MSTN mutation (OAR2:g.118150665G>A)and BMPR1B/Booroola mutation (OAR6:g.29382188A>G) on growth traits, it was observed that homozygous reference and heterozygous genotypes for both genes significantly exhibited higher BW compared to the homozygous variant genotype and Moghani pure sheep (P < 0.05, as displayed in Table 7).Furthermore, as shown in Table 7, it is noted that Moghani pure lambs exhibited a slightly higher birth weight than F2 crossbred lambs with the MSTN mutation in the homozygous variant genotype (A/A), although the difference did not reach statistical significance.In contrast, Moghani pure lambs were significantly (P < 0.05) heavier at birth compared to F2 crossbred lambs with the Booroola mutation in the homozygous variant genotype (G/G).This aligns with the findings of C ¸elikeloglu et al. [19] and Tekerli et al. [20], who conducted studies on the introgression of the MSTN mutation into Turkish Ramlıc şheep.Their findings indicated that Texel sheep with the MSTN mutation in homozygous form (A/A) exhibited significantly lower body weights in comparison to Ramlıc ¸sheep with the wild-type genotype (G/G), as well as to the first-generation backcrosses (BC1, G/G and G/A) and the second-generation backcrosses (BC2, G/G and G/A) lambs [19,20].Moreover, the A allele of the MSTN mutation OAR2:g.118150665G>Aexerted a non-significant adverse impact on live weight traits in Texel sheep [49], Norwegian White sheep [50], Turkish Ramlıc şheep [19], and New Zealand Romney sheep [51].Nevertheless, these studies did report a statistically significant positive effect on carcass and meat quality traits.
The significant interactions observed between subject effects, specifically strain × genotype and mating system × genotype (P < 0.0001), and suggest that birth weight in lambs is influenced by complex genetic and mating factors.The lower birth weight in homozygous variant genotypes could be attributed to the historical in-breeding and outcrossing practices, indicating that genetic diversity plays a crucial role in determining birth weight outcomes [52,53].These findings highlight the importance of managing genetic diversity in breeding programs to improve lamb birth weight outcomes.Interestingly, the MSTN mutation in the homozygous variant genotype exhibited significantly higher values for W3 adj , Diff1, and ADWG1 compared to other genotypes and pure Moghani sheep (P < 0.05, as shown in Table 7).Our findings align with other studies that have reported significant effects of the MSTN mutation OAR2:g.118150665G>A on the growth rate to weaning in New Zealand Romney [54] and Colored Polish Merino Sheep [55].These results indicate that the MSTN mutation OAR2:g.118150665G>A in the homozygous variant genotype has a significant impact on lamb growth until weaning, which is crucial for sheep fattening programs and ewe reproduction management.
In this study we provided additional confirmation that the Booroola/FecB does not adversely impact the growth rate, consistent with the findings reported in Garole × Malpura sheep [48].Even in the homozygous variant state, no significant differences were found for Booroola lambs in the genotypes of homozygous reference, heterozygous, and homozygous variant when compared to Moghani pure sheep for the traits of W3 adj , Diff1, and ADWG1 (P > 0.05, as shown in Table 7).We did not explore the impact of the BMPR1B/Booroola mutation on the prolificacy of crossbred lambs.Despite our thorough examination, prolificacy assessment awaits more data in future investigations.Nonetheless, previous studies have suggested that possessing one copy of the Booroola mutation (OAR_v.3.1;Chr 6: g.29382188A/G) leads to an increase in ovulation rate by 1.65 ova and in litter size by 0.9 lambs per lambing.Additionally, ewes that are homozygous (OAR_v.3.1;Chr 6: g.29382188G/G) for this mutation are estimated to experience additional increases of 1.65 ova shed and 0.3 lambs born per lambing [8,56].Importantly, Gootwine et al. [23] observed that ewes homozygous for this mutation demonstrate detrimental effects on lamb birth weight, post-weaning growth rate, and mature body weight.It has been underscored that, following the introduction of the Booroola mutation, breeding homozygous ewes is not recommended in commercial flocks due to significant lamb losses, despite the exceptionally high prolificacy observed in ewes with this genotype [8].

Conclusions
This study focused on examining how mating systems influence the performance of Moghani crossbred lambs, which are a mix of the Iranian indigenous Moghani breed and New Zealand sheep strains, including Texel and Booroola sheep.Among the mating systems employed in the development of second-generation crossbred lambs, both in-breeding and outcrossing consistently produced offspring with lean tails and woolly fleeces, emphasizing the heritability of these desirable traits.However, the examination of backcrossing progeny revealed a spectrum of tail phenotypes, ranging from short to large fat-tails, along with variations in fleece texture.We demonstrated that the utilization of paternal backcrossing emerges as a pivotal strategy for improving the growth rate and overall genetic potential of the Moghani pure breed.The deliberate choice to introgress major genes of MSTN and Booroola through this method underscores a commitment to precision and efficiency in breeding practices.The observed higher values for growth-related parameters in the MSTN mutation homozygous variant genotype indicate a lasting impact on lamb growth until weaning.This finding has significant implications for sheep fattening programs and ewe reproduction management within the Moghani sheep breed.By leveraging the advantages inherent in paternal backcrossing, specifically Texel Tamlet ram strains carrying the MSTN mutation, breeders can strategically enhance desirable traits within the Moghani breed, contributing to its resilience, adaptability, and productivity.The study emphasizes the importance of managing genetic diversity and considering both genetic markers and mating systems in sheep breeding programs.

Table 1 . Percentage rate of coat color patterns across produced strains of progenies between pure Moghani sheep and second generations of crossbred lambs (F2 and BC1). Breed Strains N Percentage rate of coat color white brown light-brown strong brown black
N: number of lambs at each group https://doi.org/10.1371/journal.pone.0301629.t001

Table 3 . Predicted means for the type of birth effects on lamb growth traits of pure Moghani sheep and second generations of crossbred lambs (F2 and BC1). Traits Type of birth SEM P Value
difference of weights at 3-6 months; ADWG1; average daily weight gain from birth to 3 months; ADWG2: average daily weight gain from 3 to 6 months.a,b: The means with the same letter in each row are not significantly different by Duncan's multiple range test at 0.05 level.https://doi.org/10.1371/journal.pone.0301629.t003

Table 4 . Predicted means for the effects of sex on lamb growth traits and fat-tail measurements in second genera- tions of crossbred lambs (F2 and BC1).
FTH: fat-tail height; FTW: fat-tail width.a,b: The means with the same letter in each column are not significantly different in Duncan's multiple range test at 0.05 level. https://doi.org/10.1371/journal.pone.0301629.t004

Table 5 . Predicted means for the mating systems on lamb growth traits and fat-tail measurements of pure Moghani sheep and second generations of crossbred lambs (F2 and BC1).
of lambs at each mating strategy; BW: birth weight; W3 adj : adjusted weight at 3 months (weaning); W6 adj : adjusted weight at 6 months; Diff1: difference of weights at birth to 3 months; Diff2: difference of weights at 3-6 months; ADWG1; average daily weight gain from birth to 3 months; ADWG2: average daily weight gain from 3 to 6 months; FTH: fat-tail height; FTW: fat-tail width.a,b: The means with the same letter in each column are not significantly different in Duncan's multiple range test at 0.05 level. https://doi.org/10.1371/journal.pone.0301629.t005

Table 6 . Predicted means for the produced strains of progenies on growth traits and fat-tail measurements between pure Moghani sheep and second generations of crossbred lambs (F2 and BC1).
FTH: fat-tail height; FTW: fat-tail width.a,b: The means with the same letter in each column are not significantly different in Duncan's multiple range test at 0.05 level.https://doi.org/10.1371/journal.pone.0301629.t006backcrossing of Texel Tamlet×Moghani (TTM) rams with Moghani pure ewes.It's worth noting that the Texel lines of Tamlet and Dalzell are associated with MyoMAX and double muscling phenotypes in New Zealand Texel sheep, both of which lead to hyperplasia or an increase in muscle fiber count

Table 7 . Predicted means for the genotype of introgressed gene on lamb growth traits between pure Moghani sheep and second generations of crossbred lambs (F2 and BC1).
3-6 months; ADWG1; average daily weight gain from birth to 3 months; ADWG2: average daily weight gain from 3 to 6 months.a,b: The means with the same letter in each column are not significantly different in Duncan's multiple range test at 0.05 level.https://doi.org/10.1371/journal.pone.0301629.t007